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Preparation of nanoparticles and nanofibers for application in anti-acne products
Tilšarová, Kamila ; Veselá, Mária (referee) ; Skoumalová, Petra (advisor)
The diploma thesis was focused on the preparation and characterization of nanoparticles and nanofibres with active substances from chosen herbs with the aim to apply this materials to the products against acne. Various types of extracts were tested on the content of polyphenols, flavonoids and antioxidation activity. These extracts were encapsulated to the liposomes and fibres of polyhydroxybutyrate. Prepared liposomes and fibres were tested mainly on antioxidation activity and antimicrobial activity against the strain Propionibacterium acnes. Then, liposomes were applied to cosmetic emulsions. These creams reported high antioxidation activity and excellent stability determined by analytical centrifugation. Prepared nanofibres also reported high antioxidation activity and antimicrobial effect as well. Finally, particles and fibres were tested in contact with human cells. In appropriate concentration, there was no cytotoxic effect and tested materials can be used in applications on problems with acne.
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Cosmetics preparations against acne
Měšťánková, Zuzana ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Hemp is a plant containing a number of active substances that can be used in cosmetics and pharmacy. The subject of this bachelor thesis is the multiplication of active substances by mixing cannabis with herbs that have anti-inflammatory effects. The theoretical part contains a description of the determined antioxidants and cannabinoids. The experimental part focuses on measuring the content of these substances in extracts created from the dry parts of herbs and hemp variety Ferimon. The work is also focused on the preparation of a cream containing cannabis and herbal extracts, which are aimed at inhibiting the formation of acne. The antimicrobial effect of creams and extracts was investigated on bacteria Propionibacterium acnes and Micrococcus luteus. The analysis of the extracts shows that the largest amount of active substances contained a mixture of mountain arnica and hemp, which also showed a good antimicrobial effect against both types of bacteria. The creams showed a low inhibitory effect due to the low content of active substances.
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Use of technical hemp in acne preparations
Žáčková, Kristýna ; Kovalčík, Adriána (referee) ; Němcová, Andrea (advisor)
Hemp belongs among important plants with a long industrial tradition. However, its first use was originally for healing. In medicine, it is mainly applied thanks to the content of many biologically active substances, such as cannabinoids. This thesis is concerned with the use of hemp in acne preparations. The theory focuses mainly on the characterization of hemp and its active substances. The other chapters deals with cosmetic preparations and acne itself, and Propionibacterium acnes as well, which is involved. Within the experimental part, the extracts of two hemp varieties (Finola and Bialobrzeskie) were characterized in terms of antioxidant and cannabinoid presence. The cosmetic preparations with hemp extract content were prepared including a complete cleansing set for acne skin, concurrently commercial products of this sort were purchased. In cosmetic preparations and the extracts their inhibitory effect against Propionibacterium acnes was studied as well. Also Micrococcus luteus, Escherichia coli and Candida glabrata were tested. The results show that dried hemp flower extracts contain the greatest amount of active substances, so they have a higher antioxidant and antimicrobial effect. The presence of individual cannabinoids differed mainly depending on the variety. All hemp extracts had effect against gram-positive bacteria, in cosmetic preparations it was lower. Nevertheless hemp antibacterial effect has been proven.
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Influence of surfactants and cosmetic polysaccharides on skin parameters and human skin microbiome
Pilipenco, Alina ; Hurčíková, Andrea (referee) ; Ilona,, Matějková (advisor)
The aim of this diploma thesis was to investigate the effect of surfactants and cosmetic polysaccharides on skin parameters and its microbiome. Three surfactants were tested to determine their effect: Sodium Dodecyl Sulfate (SDS), Cocamidopropyl Betaine (CAPB), Decylglucoside (DG). Distilled water was also used for comparison. For the next part of the experimental work were selected 6 polysaccharides: high molecular weight Hyaluronic Acid (HMW HA), very low molecular weight Hyaluronic Acid (VLMW HA), Sodium Caproyl Hyaluronate (CaproylHA), Sodium Carboxymethyl -Glucan (NaCMG), Schizophyllan and Glucomannan. For comparison, placebo and untreated control (only CAPB treatment) were also included in the tests. The first part of the work is a literature search on the assigned topic, which contains the following parts: skin anatomy and its biophysical properties, skin microbiome and its functions, description of used surfactants and polysaccharides. The experimental part is mainly focused on bioengineering methods for evaluation of skin parameters and qRT-PCR to determine the relative proportion of main bacterial species of skin microbiome. First, the effect on the CT gene of 16S rDNA was analysed, and Propionibacterium acnes and Staphylococcus epidermidis strains were selected for further analysis. In conclusion are presented an overview of all properties of selected substances and assessment of their application in cosmetics.
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Using of cannabis extracts for the acne preparation of cosmetic products
Pelánová, Lenka ; Langová, Denisa (referee) ; Němcová, Andrea (advisor)
Hemp belongs among important plants with therapeutic effects thanks to its high content of substances. This bachelor thesis brings information about the use of hemp in acne preparations. The theoretical part of this thesis focuses on the characterization of hemp and its active substances. The next chapters deal with acne and Propionibacterium acnes, which is involved in the course. For the experimental part, the extracts of two hemp varieties (Ferimon and Carmagnola) were characterized in terms of antioxidant and cannabinoid presence. The cosmetic preparations with hemp extract content were prepared and commercial products were purchased too. In both the extracts and the cosmetic preparations, their inhibitory effect against Propionibacterium acnes was observed. Micrococcus luteus, Candida glabrata and Escherichia coli were also tested. The results show that dried hemp flower extracts contain the largest amount of active substances. All hemp extracts had effect against gram-positive bacteria, in cosmetic preparations it was minimal effect or no effect.
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Study of plasma interaction with bacteria for cosmetic applications
Kocianová, Magdaléna ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis is focused on sterilization effects of low temperature plasma on grampositive, anaerobic non-sporulating bacteria Propionibacterium acnes. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost- effective and safe not only for servicing staff, but mainly for the patient. Another advantage of using low temperature plasma is the promotion of cell proliferation and wound healing. The experimental part is conducted to provide an inhibition effect of low-temperature plasma on the Propionibacterium acnes bacterium that causes a purulent inflammation. This bacteria was inoculated in various concentrations on culture soil and treated by plasma at a distance of 1 mm from the agar surface. Two plasma devices were used for plasma generation. One of them was a traditional torch discharge, the second one was based on the surface wave discharge. The microwave discharge was generated in argon at a power of 9 W, gas flow rate of 5 l/min and with water cooling to avoid thermal effects on the treated surface. From the achieved results it can be stated that the low temperature plasma has sterilization effects on a grampositive, anaerobic non-sporulating bacteriu Propionibacterium acnes.
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Antimicrobial substances from plants and their application in acne preparations
Alexová, Adéla ; Byrtusová, Dana (referee) ; Němcová, Andrea (advisor)
The aim of the bachelor thesis is a determination of selected active substances of various plants and their possible application in cosmetic products for acne. In the theoretical part, an inventory of active substances including their effects in cosmetic products has been developed. Furthermore, the work is focused on characteristics of certain types of plants, mechanisms of antimicrobial effects and the emergence and treatment of acne. In the experimental part of this work the contents of biologically active substances, antioxidant and antimicrobial activity in extracts from selected plant species are characterized, namely from flowers of Levandula angustifolia, Matricaria chamomilla, Calendula officinalis, Syzygium aromaticum, leaves of Salvia officinalis and leaves of Rosmarinus officinalis. The plant extracts are also tested in different mixtures. Plant extracts and mixtures are incorporated into a recipe of acne cosmetic products. Their inhibitory effect against Propionibacterium acnes which causes acne and against representatives G+, G- and yeast Micrococcus luteus, Escherichia coli and Candida glabrata is observed. From the results it is clear that the best products against acne are micellar water and possibly skin lotion. The biggest amount of active substances is in cosmetic products which contain Matricaria, Syzygium and Rosmarinus. Overall effect of active substances is lower in cosmetic products than in 100% extracts.
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Application of plasma and plasma activated water in cosmetics
Kocianová, Magdaléna ; Tarabová,, Barbora (referee) ; Kozáková, Zdenka (advisor)
This diploma thesis is focused on the study of the inhibitory effects of the cold atmospheric pressure plasma on the grampositive, anaerobic, non-sporulating bacteria Propionibacterium acnes. The treatment was carried out by a direct application of plasma, indirectly (using plasma-treated liquids) and also in their combination. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost-effective and safe not only for the operating staff, but especially for the patient. The experimental part focuses on the study of the direct application of plasma. Two plasma devices for the generation of microwave plasma were used. One of them was a unipolar microwave discharge torch with direct gas supply and the other was a microwave discharge with a surface wave. Discharges were generated in argon with a flow rate of 5 Slm, at a power of 9 W and 12 W. The work also deals with the indirect treatment using plasma-treated liquids. These were prepared using the pinhole discharge and the dielectric barrier discharge. In the last part, combined treatment using plasma-treated liquid and direct plasma treatment was studied. From the achieved results, it can be concluded that the sterilization effects of the plasma and the plasma-treated liquids on the Propionibacterium acnes were confirmed.
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Study of plasma interaction with bacteria for cosmetic applications
Kocianová, Magdaléna ; Krčma, František (referee) ; Kozáková, Zdenka (advisor)
This bachelor thesis is focused on sterilization effects of low temperature plasma on grampositive, anaerobic non-sporulating bacteria Propionibacterium acnes. The plasma sterilization process is fast, effective, non-toxic, environmentally friendly, cost- effective and safe not only for servicing staff, but mainly for the patient. Another advantage of using low temperature plasma is the promotion of cell proliferation and wound healing. The experimental part is conducted to provide an inhibition effect of low-temperature plasma on the Propionibacterium acnes bacterium that causes a purulent inflammation. This bacteria was inoculated in various concentrations on culture soil and treated by plasma at a distance of 1 mm from the agar surface. Two plasma devices were used for plasma generation. One of them was a traditional torch discharge, the second one was based on the surface wave discharge. The microwave discharge was generated in argon at a power of 9 W, gas flow rate of 5 l/min and with water cooling to avoid thermal effects on the treated surface. From the achieved results it can be stated that the low temperature plasma has sterilization effects on a grampositive, anaerobic non-sporulating bacteriu Propionibacterium acnes.
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Cosmetics preparations against acne
Měšťánková, Zuzana ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Hemp is a plant containing a number of active substances that can be used in cosmetics and pharmacy. The subject of this bachelor thesis is the multiplication of active substances by mixing cannabis with herbs that have anti-inflammatory effects. The theoretical part contains a description of the determined antioxidants and cannabinoids. The experimental part focuses on measuring the content of these substances in extracts created from the dry parts of herbs and hemp variety Ferimon. The work is also focused on the preparation of a cream containing cannabis and herbal extracts, which are aimed at inhibiting the formation of acne. The antimicrobial effect of creams and extracts was investigated on bacteria Propionibacterium acnes and Micrococcus luteus. The analysis of the extracts shows that the largest amount of active substances contained a mixture of mountain arnica and hemp, which also showed a good antimicrobial effect against both types of bacteria. The creams showed a low inhibitory effect due to the low content of active substances.
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